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zd105 authored Sep 30, 2019
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363 changes: 363 additions & 0 deletions RRBS.v1.1.pipeline

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91 changes: 91 additions & 0 deletions config.template
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########################################################
### CONFIGURE FILE FOR PIPELINE: RRBS.v1.1.pipeline ####
########################################################

#############
# USAGE #####
#############
# 1. Making a project running environment:
# Run a screen window for a new project,
# the screen should not be quit until the
# project run finished.
# 2. Customize your configure file:
# Modify this configure file according to
# your project.
# 3. Configure your project:
# Cat this configure file in the screen of
# your project, copy and run the output in comandline.
########################################################

#########################################################
# Project: your must modified for each new project. #####
#########################################################

# the new projet path
export Pj_path=/public/agis/gaofei_group/zhangdu/5_program/06_yongnuo3RRBS/

# reference sequence file
export ref_fa=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.fa

# reference annotation file
export ref_anno=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.refGene.txt.gz

# reference database name: UCSC, NCBI or esemble
export ref_db=UCSC

# reference cgi information file
export ref_cgi=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.cpgIslandExt.txt.gz

# reference repeats information file
export ref_rep=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.RepeatMaser.txt.gz

# samples.map file
export map=/public/agis/gaofei_group/zhangdu/5_program/06_yongnuo3RRBS/samples.map


#############################################################################################
# Software: modified only when software upgrade or run pipeline in a new computer cluster ###
#############################################################################################

# Self-developed scripts path
export myscr_path=/public/agis/gaofei_group/zhangdu/5_program/PIPELINE/RRBS/RRBS.v1.1/script

# Python 2.7.8 or later version
export python2=/public/software/bin/python

# Python 3.6.4 or later version
export python3=/public/agis/gaofei_group/zhangdu/2_soft/miniconda3/bin/python

# fastqc
export fastqc=~/mybin/fastqc

# cutadapt-1.9 or later version
export cutadapt=/public/agis/gaofei_group/zhangdu/2_soft/cutadapt-1.9/bin/cutadapt

# Rscript 3.4 or later version
# R package reqiured: [ ggplot2;vioplot;ade4;pvclust;seqLogo;corrplot; ]
export Rscript=/public/home/zhangdu/src/R-3.4.2/builddir/bin/Rscript

## bsmap, version >= 2.73
export bsmap=/public/agis/gaofei_group/zhangdu/2_soft/bsmap-2.73/

# metilene dir, version >= 0.2-6
export metilene=/public/agis/gaofei_group/zhangdu/2_soft/metilene_v0.2-6/

# EAGE program path
export EAGE=/public/agis/gaofei_group/zhangdu/3_script/EAGE/

# bedtools
export bedtools=/public/agis/gaofei_group/zhangdu/2_soft/bedtools2/bin/bedtools

###############
### Database ##
###############

# lambda DNA sequence
export Control=/public/agis/gaofei_group/zhangdu/3_script/pipeline/Methylome/WGBS-seq-1.0/Example/reference/Control.fa


###########
####END####
###########
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