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######################################################## | ||
### CONFIGURE FILE FOR PIPELINE: RRBS.v1.1.pipeline #### | ||
######################################################## | ||
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############# | ||
# USAGE ##### | ||
############# | ||
# 1. Making a project running environment: | ||
# Run a screen window for a new project, | ||
# the screen should not be quit until the | ||
# project run finished. | ||
# 2. Customize your configure file: | ||
# Modify this configure file according to | ||
# your project. | ||
# 3. Configure your project: | ||
# Cat this configure file in the screen of | ||
# your project, copy and run the output in comandline. | ||
######################################################## | ||
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######################################################### | ||
# Project: your must modified for each new project. ##### | ||
######################################################### | ||
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# the new projet path | ||
export Pj_path=/public/agis/gaofei_group/zhangdu/5_program/06_yongnuo3RRBS/ | ||
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# reference sequence file | ||
export ref_fa=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.fa | ||
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# reference annotation file | ||
export ref_anno=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.refGene.txt.gz | ||
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# reference database name: UCSC, NCBI or esemble | ||
export ref_db=UCSC | ||
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# reference cgi information file | ||
export ref_cgi=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.cpgIslandExt.txt.gz | ||
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# reference repeats information file | ||
export ref_rep=/public/agis/gaofei_group/zhangdu/4_DB/01_genome/mmu/UCSC/mmu10.RepeatMaser.txt.gz | ||
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# samples.map file | ||
export map=/public/agis/gaofei_group/zhangdu/5_program/06_yongnuo3RRBS/samples.map | ||
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############################################################################################# | ||
# Software: modified only when software upgrade or run pipeline in a new computer cluster ### | ||
############################################################################################# | ||
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# Self-developed scripts path | ||
export myscr_path=/public/agis/gaofei_group/zhangdu/5_program/PIPELINE/RRBS/RRBS.v1.1/script | ||
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# Python 2.7.8 or later version | ||
export python2=/public/software/bin/python | ||
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# Python 3.6.4 or later version | ||
export python3=/public/agis/gaofei_group/zhangdu/2_soft/miniconda3/bin/python | ||
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# fastqc | ||
export fastqc=~/mybin/fastqc | ||
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# cutadapt-1.9 or later version | ||
export cutadapt=/public/agis/gaofei_group/zhangdu/2_soft/cutadapt-1.9/bin/cutadapt | ||
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# Rscript 3.4 or later version | ||
# R package reqiured: [ ggplot2;vioplot;ade4;pvclust;seqLogo;corrplot; ] | ||
export Rscript=/public/home/zhangdu/src/R-3.4.2/builddir/bin/Rscript | ||
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## bsmap, version >= 2.73 | ||
export bsmap=/public/agis/gaofei_group/zhangdu/2_soft/bsmap-2.73/ | ||
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# metilene dir, version >= 0.2-6 | ||
export metilene=/public/agis/gaofei_group/zhangdu/2_soft/metilene_v0.2-6/ | ||
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# EAGE program path | ||
export EAGE=/public/agis/gaofei_group/zhangdu/3_script/EAGE/ | ||
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# bedtools | ||
export bedtools=/public/agis/gaofei_group/zhangdu/2_soft/bedtools2/bin/bedtools | ||
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############### | ||
### Database ## | ||
############### | ||
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# lambda DNA sequence | ||
export Control=/public/agis/gaofei_group/zhangdu/3_script/pipeline/Methylome/WGBS-seq-1.0/Example/reference/Control.fa | ||
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########### | ||
####END#### | ||
########### |
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