Systemin perception and signaling rely on the systemin receptor SYR1. However, SYR1-dependent signaling, and how systemin signaling differs from other peptide-mediated signaling pathways, is largely unknown. This experiment contained WT and syr1(systemin receptor knockout) cell culture treated by systemin.Samples were harvested at 0,1,2,5,15,45 min and each time point had 6 biological replicates.

Script folder contains all analyses(code) done in R; Data folder has results from analyses(scripts folder) as well as tomato genome annotation ITAG3.2; Figure folder has all the plots present in the paper.

PPase linear regression plot (Figure2a) is a linear regression plot showing the phosphate release progress curves of different phosphatases.

Run using R version 4.4.0;Operating system is macOS Monterey(Version 12.7.6).

R packages including limma(3.60.4); ggplot2(3.5.1); tidyverse(2.0.0); missForest(1.5); stringr(1.5.1); factoextra(1.0.7); reshape2(1.4.4); hypeR(2.2.0); ggpubr(0.6.0); viridis(0.6.5).Packages should be compatible with Windows, Mac, and Linux operating systems.

If files in Data folder can not be viewed on-site, please press the [download] button at the upper right corner of the file of interest.