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added to FAQs for tool documentation
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ChristelKrueger committed Dec 12, 2023
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Expand Up @@ -33,6 +33,11 @@ A: Librarian is specifically for Illumina samples. Other sequencing technologies
A: No. Librarian will extract base compositions from a random selection of 100 000 reads. Only the compositions will be submitted to the web server.


### Q: I have paired-end data. Which read should I upload?

A: This depends on the library type. Consider the following questions: Is one of your reads and index read (e. g. read 1 in 10X scRNA-seq)? Don't use that one as it won't be informative. Will your reads be substantially different? For example, for standard ChIP-seq, both reads will be very similar, whereas for PBAT libraries they will be very different. The compositions reference database was constructed on read 1 for paired end data. Therefore, if none of your reads is an index read, stick with read 1.


### Q: Do you collect any data from samples submitted to the web app?

A: No.
Expand All @@ -45,3 +50,5 @@ A: This seems to be an issue outside Librarian which we found happens on some Ma





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