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update links to updated lectures throughout
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4 changes: 2 additions & 2 deletions _posts/0000-01-01-Intro_to_RNAseq.md
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### Lectures

* [Introduction to instructors](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/full/RNASeq_Module0_Introductions.pdf)
* [Introduction to cloud computing](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/full/RNASeq_Module0_CloudComputing.pdf)
* [Introduction to instructors](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/full/RNASeq_Module0_Introductions.pdf)
* [Introduction to cloud computing](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/full/RNASeq_Module0_CloudComputing.pdf)
6 changes: 3 additions & 3 deletions _posts/0001-01-01-Intro_to_Inputs.md
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### Lecture
* [Introduction to RNA sequencing lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/full/RNASeq_Module1_IntrotoRNA.pdf)
* [FASTA/FASTQ/GTF formats mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf)
* [Indexing mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_01_02_Indexing.pdf)
* [Introduction to RNA sequencing lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/full/RNASeq_Module1_IntrotoRNA.pdf)
* [FASTA/FASTQ/GTF formats mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf)
* [Indexing mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_01_02_Indexing.pdf)
2 changes: 1 addition & 1 deletion _posts/0001-02-01-Reference_Genomes.md
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***

### FASTA/FASTQ/GTF mini lecture
If you would like a refresher on common file formats such as FASTA, FASTQ, and GTF files, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf) briefly covering these.
If you would like a refresher on common file formats such as FASTA, FASTQ, and GTF files, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf) briefly covering these.

### Obtain a reference genome from Ensembl, iGenomes, NCBI or UCSC.

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2 changes: 1 addition & 1 deletion _posts/0001-03-01-Annotations.md
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***

### FASTA/FASTQ/GTF mini lecture
If you would like a refresher on common file formats such as FASTA, FASTQ, and GTF files, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf) briefly covering these.
If you would like a refresher on common file formats such as FASTA, FASTQ, and GTF files, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_01_01_FASTA_FASTQ_GTF.pdf) briefly covering these.


### Obtain Known Gene/Transcript Annotations
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2 changes: 1 addition & 1 deletion _posts/0001-04-01-Indexing.md
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***

### Indexing mini lecture
If you want a refresher on indexing, we have made an [indexing mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_01_02_Indexing.pdf) available.
If you want a refresher on indexing, we have made an [indexing mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_01_02_Indexing.pdf) available.

### Create a HISAT2 index
Create a HISAT2 index for chr22 and the ERCC spike-in sequences. HISAT2 can incorporate exons and splice sites into the index file for alignment. First create a splice site file, then an exon file. Finally make the aligner FM index.
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10 changes: 5 additions & 5 deletions _posts/0002-01-01-Intro_to_Alignment.md
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* BAM read counting and determination of variant allele expression status

### Lectures
* [Introduction to alignment mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_01_Alignment.pdf)
* [Alignment/Assembly/kmer mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf)
* [SAM/BAM/BED formats mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_03_SAM_BAM_BED.pdf)
* [Introduction to IGV lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/full/RNASeq_Module2_IGV_Tutorial_Brief.pdf)
* [Alignment QC mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_04_alignmentQC.pdf)
* [Introduction to alignment mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_01_Alignment.pdf)
* [Alignment/Assembly/kmer mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf)
* [SAM/BAM/BED formats mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_03_SAM_BAM_BED.pdf)
* [Introduction to IGV lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/full/RNASeq_Module2_IGV_Tutorial_Brief.pdf)
* [Alignment QC mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_04_alignmentQC.pdf)
4 changes: 2 additions & 2 deletions _posts/0002-03-01-Alignment.md
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---

### Alignment mini lecture
If you would like a refresher on alignment, we have created an [alignment mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_01_Alignment.pdf).
If you would like a refresher on alignment, we have created an [alignment mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_01_Alignment.pdf).

We have also provided a mini lectures describing the differences between [alignment, assembly, and pseudoalignment](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf) and describing [sam, bam, and bed file formats](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_03_SAM_BAM_BED.pdf).
We have also provided a mini lectures describing the differences between [alignment, assembly, and pseudoalignment](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf) and describing [sam, bam, and bed file formats](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_03_SAM_BAM_BED.pdf).

### HISAT2 alignment
Perform alignments with HISAT2 to the genome and transcriptome.
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2 changes: 1 addition & 1 deletion _posts/0002-06-01-Alignment_QC.md
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***

### Alignment QC mini lecture
If you would like a refresher on alignment QC, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_04_alignmentQC.pdf) briefly covering the topic.
If you would like a refresher on alignment QC, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_04_alignmentQC.pdf) briefly covering the topic.

### Use samtools and FastQC to evaluate the alignments
Use `samtools view` to see the format of a SAM/BAM alignment file
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6 changes: 3 additions & 3 deletions _posts/0003-01-01-Intro_to_Expression.md
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* Downstream interpretation of expression and differential estimates

### Lectures
* [Abundance Estimations lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_01_AbundanceEstimation.pdf)
* [HTSEQ lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_02_HTSEQ.pdf)
* [Differential expression lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_03_DifferentialExpression.pdf)
* [Abundance Estimations lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_01_AbundanceEstimation.pdf)
* [HTSEQ lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_02_HTSEQ.pdf)
* [Differential expression lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_03_DifferentialExpression.pdf)
4 changes: 2 additions & 2 deletions _posts/0003-02-01-Expression.md
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***

### Expression mini lecture
If you would like a refresher on expression and abundance estimations, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_01_AbundanceEstimation.pdf).
If you would like a refresher on expression and abundance estimations, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_01_AbundanceEstimation.pdf).

### Use Stringtie to generate expression estimates from the SAM/BAM files generated by HISAT2 in the previous module

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#### Mini-lecture

For more on the differences between abundance estimates like FPKM and count data with HTSeq-count, see this [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_02_HTSEQ.pdf).
For more on the differences between abundance estimates like FPKM and count data with HTSeq-count, see this [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_02_HTSEQ.pdf).

***

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2 changes: 1 addition & 1 deletion _posts/0003-03-01-Differential_Expression.md
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### Differential Expression mini lecture
If you would like a brief refresher on differential expression analysis, please refer to the [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_03_03_DifferentialExpression.pdf).
If you would like a brief refresher on differential expression analysis, please refer to the [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_03_03_DifferentialExpression.pdf).


### Ballgown DE Analysis
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4 changes: 2 additions & 2 deletions _posts/0004-01-01-Intro_to_Alignment_Free.md
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* Abundance estimation and differential expression analysis with Kallisto and Sleuth

### Lectures
* [Psuedo-alignment mini-lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_04_01_AlignmentFreeKallisto.pdf)
* [Differences between alignment, assembly, and pseudoalignment mini-lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf).
* [Psuedo-alignment mini-lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_04_01_AlignmentFreeKallisto.pdf)
* [Differences between alignment, assembly, and pseudoalignment mini-lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf).
4 changes: 2 additions & 2 deletions _posts/0004-02-01-Alignment_Free_Kallisto.md
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### Kallisto mini lecture

If you would like a refresher on Kallisto, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_04_01_AlignmentFreeKallisto.pdf) briefly covering the topic.
We have also made a mini lecture describing the differences between [alignment, assembly, and pseudoalignment](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf).
If you would like a refresher on Kallisto, we have made a [mini lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_04_01_AlignmentFreeKallisto.pdf) briefly covering the topic.
We have also made a mini lecture describing the differences between [alignment, assembly, and pseudoalignment](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_02_02_Alignment_vs_Assembly_vs_Kmer.pdf).


***
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2 changes: 1 addition & 1 deletion _posts/0005-01-01-Intro_to_Isoform_Analysis.md
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* Visualize junction counts and StringTie assembled transcripts in IGV

### Lecture
* [Alternative Splicing lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cbw/2023/mini/RNASeq_MiniLecture_05_01_AlternativeSplicingAnalysis.pdf)
* [Alternative Splicing lecture](https://github.com/griffithlab/rnabio.org/blob/master/assets/lectures/cshl/2023/mini/RNASeq_MiniLecture_05_01_AlternativeSplicingAnalysis.pdf)
9 changes: 9 additions & 0 deletions _posts/0009-04-01-Lectures.md
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* [2013 CBW course lectures](https://www.youtube.com/playlist?list=PL3izGL6oi0S_N63aEd9vLnFM3pNYt9NGQ) (YouTube)


### Advanced Sequencing Technologies & Bioinformatics Analysis (November 6-19, 2023)

Organized by [Cold Spring Harbor Laboratory (CSHL)](http://www.cshl.edu/) - [Course Site](https://meetings.cshl.edu/courses.aspx?course=c-seqtec&year=23)

**Presentations:**

TBA


### CBW RNA-seq analysis (July 17-19th, 2023. Toronto, ON, Canada))

Organized by [Canadian Bioinformatics Workshops (CBW)](https://bioinformatics.ca/) - [Course Site](https://bioinformaticsdotca.github.io/RNA_2021)
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