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41 changes: 41 additions & 0 deletions CITATION.cff
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# This CITATION.cff file was generated with cffinit.
# Visit https://bit.ly/cffinit to generate yours today!

cff-version: 1.2.0
title: >-
Single-cell RNA sequencing (scRNA-seq) Data Processing &
Visualization Workflow
message: >-
If you use this software, please cite it using the
metadata from this file.
type: software
authors:
- given-names: Stephan
family-names: Reichl
orcid: 'https://orcid.org/0000-0001-8555-7198'
affiliation: CeMM Research Center for Molecular Medicine
- given-names: Christoph
family-names: Bock
orcid: 'https://orcid.org/0000-0001-6091-3088'
affiliation: CeMM Research Center for Molecular Medicine
identifiers:
- type: doi
value: 10.5281/zenodo.10679327
description: >-
This DOI represents all versions, and will always
resolve to the latest one.
repository-code: 'https://github.com/epigen/scrnaseq_processing_seurat/'
url: 'https://epigen.github.io/scrnaseq_processing_seurat/'
abstract: >-
A Snakemake workflow for processing and visualizing
(multimodal) sc/snRNA-seq data generated with 10X Genomics
Kits or in the MTX matrix file format powered by the R
package Seurat.
keywords:
- scRNA-seq
- Visualization
- Multimodal
- Bioinformatics
- Workflow
- Snakemake
license: MIT
8 changes: 6 additions & 2 deletions README.md
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[![DOI](https://zenodo.org/badge/468752490.svg)](https://zenodo.org/doi/10.5281/zenodo.10679327)

# Single-cell RNA sequencing (scRNA-seq) Data Processing & Visualization Snakemake Workflow

sc/snRNA-seq Data Processing & Visualization Snakemake Workflow powered by Seurat
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**If you use this workflow in a publication, don't forget to give credits to the authors by citing the URL of this (original) repository (and its DOI, see Zenodo badge above -> coming soon).**

**If you use this workflow in a publication, please don't forget to give credits to the authors by citing it using this DOI [10.5281/zenodo.10679327](https://zenodo.org/doi/10.5281/zenodo.10679327).**

![Workflow Rulegraph](./workflow/dags/rulegraph.svg)

Table of contents
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**Visualization.** To visualize the metadata after each processing step inspectdf (ver) [ref] was used. For the visualization of expression, multimodal [X] data and calculated metadata like module scores, the Seurat functions RidgePlot for ridge plots, VlnPlot for violin plots, DotPlot for dot plots and DoHeatmap for heatmaps were used.

**The processing, analysis and visualizations described here were performed using a publicly available Snakemake [ver] (ref) workflow [ref - cite this workflow here].**
**The processing, analysis and visualizations described here were performed using a publicly available Snakemake [ver] (ref) workflow [[10.5281/zenodo.10679327](https://zenodo.org/doi/10.5281/zenodo.10679327)].**


# Features
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# Links
- [GitHub Repository](https://github.com/epigen/scrnaseq_processing_seurat/)
- [GitHub Page](https://epigen.github.io/scrnaseq_processing_seurat/)
- [Zenodo Repository (coming soon)]()
- [Zenodo Repository](https://zenodo.org/doi/10.5281/zenodo.10679327)
- [Snakemake Workflow Catalog Entry](https://snakemake.github.io/snakemake-workflow-catalog?usage=epigen/scrnaseq_processing_seurat)

# Resources
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