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readme.txt
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###---------------------------------------------------------------------------------------------------------------------
### Affy + limma + GOstat pipeline for analysing affymetrix expression array data.
### 06/25/2015 Xing Tang, The Ohio State University Comprehensive Cancer Center
###---------------------------------------------------------------------------------------------------------------------
How to use this pipeline?
1. customize you parameters by modifying sample_desc.txt file
2. type "Rscript do_rma_limma_GO.R" to get the results
3. check results/ folder. Four tables are generated.
a. AllExp.xls: log2 signal generated by rma function from affy
b. DEG.xls: differential expression analysis results from limma
c. GO.xls: enriched GO terms from GOstat package
*Note
1. you have to modify the do_rma_limma_GO.R scripts to fit the pipeline to multiple groups comparison