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something wrong with chain file T_T #18

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dingyigithub opened this issue Oct 25, 2024 · 0 comments
Open

something wrong with chain file T_T #18

dingyigithub opened this issue Oct 25, 2024 · 0 comments

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@dingyigithub
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Hi, Wenjie
Thanks for the amazing tool!
These days I‘ve been trying to convert a PAF file to a CHAIN file using wgatools. When I run the wgatools paf2chain, the chain file is successfully generated. But when I tried to apply liftOver to perform coordinate lift over on a bed file by:

liftOver bundle1_T2T_loc.bed mPanTro3.pri.cur.chr5_hsa6.humanT2T_chr6.chain bundle1_Primate-T2T_loc.bed bundle1_Primate-T2T_loc.unmapp.bed

it arises an error:
q end mismatch 122108 vs 183202006 line 42 of mPanTro3.pri.cur.chr5_hsa6.humanT2T_chr6.chain.
Is it due to wgatools or just due to something wrong with my paf file? I'm not quite sure about these stuff. The paf, chain and bed file used have been attached here. Thx for your help😘
issue.zip

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