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Can zUMIs be used for Smart-Seq3 on only one sample #407

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realzhang opened this issue Jan 19, 2025 · 0 comments
Open

Can zUMIs be used for Smart-Seq3 on only one sample #407

realzhang opened this issue Jan 19, 2025 · 0 comments

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@realzhang
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I've performed Smart-seq3 on only one type of cells (~hundreds of cells from FACS), so actually only one BC was used.
But when I ran zUMIs on the fastq files:

Error in uik(bccount$cellindex, bccount$cs/1000) : 
  Method is not applicable for such a small vector. Please give at least a 5 numbers vector
Calls: cellBC -> .cellBarcode_unknown -> .FindBCcut -> uik
Execution halted
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