You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi,
I tried to use NOVO for the assembly of the primate mitochondrial genome,
I follow all the instructions, but it doesn't seem to work properly.
Can you help?
run command:
perl /home/zl/software/NOVOplasty-master/NOVOPlasty4.3.1.pl -c config.txt
config.txt:
Project:
Project name = FJ559
Type = mito
Genome Range = 15000-17000
K-mer = 30
Max memory =
Extended log = 0
Save assembled reads = yes
Seed Input = /home/zl/spider/zlbz/snp_mul10/download_cleandata/fj559/sequence_cytb.fasta
Extend seed directly = no
Reference sequence = /home/zl/spider/zlbz/snp_mul10/download_cleandata/fj559/sequence.fasta
Variance detection =
You probaply ran out of memory, most systems will kill the script, but seems some just keep it hanging
6 days is very long, should be between few minutes to few hours depending on the size.
If you have a machine with limited memory, best to use the max memory option, make sure you give a value bit below the max available on your machine. It will subsample the dataset, but this ok WGS dataset usually have way too much coverage anyway...
Hi,
I tried to use NOVO for the assembly of the primate mitochondrial genome,
I follow all the instructions, but it doesn't seem to work properly.
Can you help?
run command:
perl /home/zl/software/NOVOplasty-master/NOVOPlasty4.3.1.pl -c config.txt
config.txt:
Project:
Project name = FJ559
Type = mito
Genome Range = 15000-17000
K-mer = 30
Max memory =
Extended log = 0
Save assembled reads = yes
Seed Input = /home/zl/spider/zlbz/snp_mul10/download_cleandata/fj559/sequence_cytb.fasta
Extend seed directly = no
Reference sequence = /home/zl/spider/zlbz/snp_mul10/download_cleandata/fj559/sequence.fasta
Variance detection =
Dataset 1:
Read Length = 150
Insert size = 300
Platform = illumina
Single/Paired = PE
Combined reads =
Forward reads = /home/zl/spider/zlbz/snp_mul10/download_cleandata/SRR5019559_clean_1.fastq.gz
Reverse reads = /home/zl/spider/zlbz/snp_mul10/download_cleandata/SRR5019559_clean_2.fastq.gz
Store Hash =
Heteroplasmy:
MAF =
HP exclude list =
PCR-free =
Optional:
Insert size auto = yes
Use Quality Scores = no
Reduce ambigious N's =
Output path = /home/zl/spider/zlbz/snp_mul10/download_cleandata/fj559/
Project:
The output log has always been:
Reading Input......OK
Scan reference sequence......OK
Building Hash Table...
It's been 6 days
Thanks
The text was updated successfully, but these errors were encountered: