NTR: [transcription pausing by RNA polymerase II]

[sylvainpoux]

Hi GO,

• While working on eralry steps of transcription, I realzed that there is not term for transcription pausing by RNA polymerase II, a step that is now recognized as an integral part of transcription. I will then reannotate the DSIF and NELF complexes, that are currently curated as inhibitors of transcription elongation, which is not exact, since they preceed transcription elongation. This step is also essential for RNA polymerase II transcription elongation surveillance (see ticket NTR: [RNA polymerase II transcription elongation surveillance] #29516)

Thanks

Sylvain

Term: transcription pausing by RNA polymerase II
Definition: A transcription halt following transcription initiation but prior to elongation, during which RNA polymerase II pauses approximately 20-60 nucleotides downstream of the transcriptional start site before proceeding into productive elongation.
Synonym: Promoter proximal pausing by RNA polymerase II
Child of GO:0006366 transcription by RNA polymerase II
PMID:35816930
PMID:33271312

We should also change the definition of transcription elongation
GO:0006368 transcription elongation by RNA polymerase II
FROM:
The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.
TO:
The extension of an RNA molecule after transcription pausing and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.