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Here, I collect some things that I found confusing. I think it never hurts to deconfuse the user as much as possible (even though many things could be clear I guess, but why not make it easy)
group in filter_parameters. → Add comment: group is a column in annotation
reCombat_parametersnorm_methods: Which methods can I use from above how? Default ["CQN", "TMM"] suggests that I use uppercase letters, but I have to guess. e.g., would this work? ["TMM", "VOOM", "RLE", "none"]? → provide all options in comment as list if possible? Or explain how they come about? → this one was also hard to resolve with looking into the code, e.g., for "none" in edgeR_parameters: method: I think I should use "CPM" in my case (edgeR_parameters: quantification:) instead of "none"
Since you collect best practices here that also include knowledge, some words on whether it makes sense to use VOOM and then reCombat (or similar combinations) would be nice → it's not in the defaults and I don't understand why.
if you are using limma-trend you start with normalized data, that's why I was not thinking of voom. voom I just added so the user gets a feeling how the data actually looks inside of a limma-voom approach.
The text was updated successfully, but these errors were encountered:
Here, I collect some things that I found confusing. I think it never hurts to deconfuse the user as much as possible (even though many things could be clear I guess, but why not make it easy)
group
infilter_parameters
. → Add comment:group is a column in annotation
reCombat_parameters
norm_methods
: Which methods can I use from above how? Default["CQN", "TMM"]
suggests that I use uppercase letters, but I have to guess. e.g., would this work?["TMM", "VOOM", "RLE", "none"]
? → provide all options in comment as list if possible? Or explain how they come about? → this one was also hard to resolve with looking into the code, e.g., for "none" inedgeR_parameters: method:
I think I should use "CPM" in my case (edgeR_parameters: quantification:
) instead of "none"The text was updated successfully, but these errors were encountered: