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Help with ClustalW.. #2227

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AlliKoehle opened this issue May 19, 2020 · 0 comments
Open

Help with ClustalW.. #2227

AlliKoehle opened this issue May 19, 2020 · 0 comments

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@AlliKoehle
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I am trying to run an alignment with ClustalW, but I keep receiving this error...

Traceback (most recent call last):
File "/macqiime/anaconda/bin/align_seqs.py", line 211, in
main()
File "/macqiime/anaconda/bin/align_seqs.py", line 208, in main
log_path=log_path)
File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/align_seqs.py", line 123, in call
log_path=log_path, *args, **kwargs)
File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/util.py", line 259, in call
result = self.getResult(*args, **kwargs)
File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/align_seqs.py", line 117, in getResult
result = module.align_unaligned_seqs(seqs, params=params)
File "/macqiime/anaconda/lib/python2.7/site-packages/bfillings/clustalw.py", line 584, in align_unaligned_seqs
app = Clustalw(InputHandler='_input_as_multiline_string',params=params)
File "/macqiime/anaconda/lib/python2.7/site-packages/burrito/util.py", line 201, in init
self._error_on_missing_application(params)
File "/macqiime/anaconda/lib/python2.7/site-packages/burrito/util.py", line 468, in _error_on_missing_application
"Is it in your path?" % command)
burrito.util.ApplicationNotFoundError: Cannot find clustalw. Is it installed? Is it in your path?

This is my first time using Qiime, so I unsure of what I should to doing to resolve this error. Can anyone help with this?

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