diff --git a/_resources/mpxv/mpxv-multiplex-pcr-protocol-2500.docx b/_resources/mpxv/mpxv-multiplex-pcr-protocol-2500.docx new file mode 100644 index 0000000..dc1d72f Binary files /dev/null and b/_resources/mpxv/mpxv-multiplex-pcr-protocol-2500.docx differ diff --git a/_resources/mpxv/mpxv-multiplex-pcr-protocol-400.docx b/_resources/mpxv/mpxv-multiplex-pcr-protocol-400.docx new file mode 100644 index 0000000..17f6dcd Binary files /dev/null and b/_resources/mpxv/mpxv-multiplex-pcr-protocol-400.docx differ diff --git a/_resources/mpxv/mpxv-sequencing-resources.md b/_resources/mpxv/mpxv-sequencing-resources.md index 825e9e5..0279142 100644 --- a/_resources/mpxv/mpxv-sequencing-resources.md +++ b/_resources/mpxv/mpxv-sequencing-resources.md @@ -36,6 +36,10 @@ content='**Overview:** This document is part of the Mpox virus (MPXV) sequencing ## MPXV sequencing +> ARTIC/INRB Clade-independent Primer Schemes +: 2500 bp primer scheme [Clade-I reference](https://labs.primalscheme.com/detail/artic-inrb-mpox/2500/v1.0.0-cladeia/?q=mpox), [Clade II reference](https://labs.primalscheme.com/detail/artic-inrb-mpox/2500/v1.0.0-cladeib/?q=mpox), [/mpxv/mpox-multiplex-pcr-protocol-2500.docx](PCR amplification protocol) +: 400 bp primer scheme [Clade-I reference](https://labs.primalscheme.com/detail/artic-inrb-mpox/400/v1.0.0-cladeia/?q=mpox), [Clade II reference](https://labs.primalscheme.com/detail/artic-inrb-mpox/400/v1.0.0-cladeib/?q=mpox), [/mpxv/mpox-multiplex-pcr-protocol-400.docx](PCR amplification protocol) + > Tiled amplicon sequencing protocol and primer scheme for MPXV : Chen *et al.* Yale amplicon sequencing protocol \- [dx.doi.org/10.17504/protocols.io.5qpvob1nbl4o/v4](http://dx.doi.org/10.17504/protocols.io.5qpvob1nbl4o/v4) : This protocol is a primer scheme and validation for MPXV of the [ARTIC SARS-CoV-2 tiled amplicon protocol](https://dx.doi.org/10.17504/protocols.io.bp2l6n26rgqe/v4)