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DESCRIPTION
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Package: scruff
Title: Single Cell RNA-Seq UMI Filtering Facilitator (scruff)
Version: 1.3.6
Date: 2019-08-08
Authors@R: c(person("Zhe", "Wang",
email = "[email protected]",
role = c("aut", "cre")),
person("Junming", "Hu",
email = "[email protected]",
role = c("aut")),
person("Joshua", "Campbell",
email = "[email protected]",
role = c("aut")))
Description: A pipeline which processes single cell RNA-seq (scRNA-seq) reads
from CEL-seq and CEL-seq2 protocols. Demultiplex scRNA-seq FASTQ files,
align reads to reference genome using Rsubread, and generate UMI filtered
count matrix. Also provide visualizations of read alignments and pre- and
post-alignment QC metrics.
Depends: R (>= 3.5.0)
License: MIT + file LICENSE
Encoding: UTF-8
LazyData: false
RoxygenNote: 6.1.1
VignetteBuilder: knitr
Imports: data.table,
GenomicAlignments,
GenomicFeatures,
GenomicRanges,
Rsamtools,
ShortRead,
parallel,
plyr,
BiocGenerics,
BiocParallel,
S4Vectors,
AnnotationDbi,
Biostrings,
methods,
ggplot2,
ggthemes,
scales,
GenomeInfoDb,
stringdist,
ggbio,
rtracklayer,
SingleCellExperiment,
SummarizedExperiment
Suggests:
BiocStyle,
knitr,
rmarkdown,
Rsubread,
testthat
biocViews:
Software,
Technology,
Sequencing,
Alignment,
RNASeq,
SingleCell,
WorkflowStep,
Preprocessing,
QualityControl,
Visualization,
ImmunoOncology
BugReports: https://github.com/campbio/scruff/issues