spades-core: /spades/assembler/src/common/assembly_graph/core/debruijn_data.hpp:38: void debruijn_graph::CoverageData::inc_coverage(int): Assertion `value >= 0 || coverage_ > unsigned(-value)' failed.

[Amandkaur]

Description of bug

Greetings!
Thank you for the amazing tool. I am having trouble in assembling shotgun metagenomic raw reads of some of the samples. I am not sure what underlying issue is and how to resolve. I have tried different memory allocations and k-mer sizes but every time it terminates at the same step.

spades.log

spades.log

params.txt

params.txt

SPAdes version

3.13.0

Operating System

Linux-4.4.0-210-generic-x86_64-with-debian-stretch-sid

Python Version

3.7.9

Method of SPAdes installation

manual

No errors reported in spades.log

• Yes

[asl]

You are using ancient version of SPAdes. Does the issue reproduce on the latest SPAdes 4.0? Likely your reads contain over-represented k-mer (e.g. an untrimmed adapter or something like this).