RNA-seq and RIBO-seq do not have replicates.

[junjunlab]

Thank you for your great package to analysis differential translation events on ribo-seq data. I am using your package to analysis my own data,but my rnaseq and riboseq data do not have replicates,how can i use this package to find differential translation genes ? I am looking forward to your reply!

[soniachothani]

Hi,

Thank you for your query. We recommend, "At least three biological replicates per condition or group are recommended for robust analysis of differential transcription, translation, and translational efficiency", Chothani et al, Curr. Prot. Molecular Biology, 2019. You can use the raw R script (DTEG.R) line-by-line to explore the individual samples (for PCA, count distribution etc,.) but without replicates you won't be able to get any reliable statistical inferences on differential analysis, unfortunately.

Best wishes,
Sonia