Error Added sequences to a total of 0 reads.

[luckyvivi]

Hi, I extracted one of my fast5 files for test but meet an error. I followed all the process on your website, except changing the 'cat fast5s_guppy/.fastq > fast5s_guppy.fastq' command to 'cat fast5s_guppy/pass/.fastq > fast5s_guppy.fastq'.

Below are my commands, could you please help me see what went wrong? Thanks!

[PengNi]

Hi @luckyvivi , there may be 2 reasons.

1. multi-read FAST5 format. Tombo doesn't accept multi-read FAST5s, so the FAST5 files has to be converted to single-read format. Check readme of deepsignal-plant and readme of tombo.
2. The VBZ compression issue. please see known issues of deepsignal-plant.

Thank you for using this tool.

Best,
Peng