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Develop tutorial for growth curve analysis #14

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3 of 7 tasks
stephan-koenig opened this issue Oct 8, 2020 · 0 comments
Open
3 of 7 tasks

Develop tutorial for growth curve analysis #14

stephan-koenig opened this issue Oct 8, 2020 · 0 comments
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@stephan-koenig
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stephan-koenig commented Oct 8, 2020

  • This module will be taught on Nov 23.

  • On branch growth-curve.

  • Place tutorial in inst/tutorials/growth_curve.

  • Create a data table in well_information.csv format from well_information.png with the following columns:

    • student: use only generic names, i.e. student A, etc.
    • blank: TRUE for blanks, FALSE for wells with bacteria (i.e. wells with indicated OD, ignore value).
    • media: LB for 3133, and MM (i.e "minimal media") for 3145.
  • Delete well_information.pngfrom repo after conversion to .csv.

  • Write instructions for students on how to transcribe the well_information.png.

  • Create learnr tutorial growth_curve.Rmd. Use tidyverse functions were sensible to:

    • Import the data (well information and growth data growth_data.csv) into R.
    • Join the two data tables.
    • Plot the growth curve for each well (ggplot).
    • Plot average growth curve with error bars for each treatment (i.e. media).
    • Determine the slope during exponential growth (still looking into how to do so).
    • Test if two conditions grow at significantly different speeds (unsure what statistical test is appropriate).
  • Flesh out Rmd to provide context to individual steps.

  • Integrate growth_data.csv as an external data set.

  • Create a download button, so a local copy of datasets is saved.

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