Ancestor node sequence question

[bighawkin]

I have a command that is still running. its evolutionary tree is as follows. Sorry, I failed to upload the visualization image.

[2025-01-06T08:46:04+0800] [MainThread] [I] [toil.statsAndLogging] Tree: (Chenghua:0.002007248921,Meishan:0.001989041079,(((((Norwegian_Landrace:0.001387818346,Duroc:0.001382871654)Anc06:0.0001757475742,(Pietrain:0.001392569191,Large_White:0.001043390809)Anc07:0.0002192699258)Anc04:0.001301383252,(((((((((Canis_lupus:0.06159129412,Felis_catus:0.06412370588)Anc17:0.014393375,Oryctolagus_cuniculus:0.099973625)Anc16:0.005788016667,Homo_sapiens:0.07980748333)Anc15:0.003457919643,Mus_musculus:0.1035495804)Anc13:0.005976822115,(Bos_taurus:0.02936181111,Ovis_aries:0.03196458889)Anc14:0.04737097788)Anc12:0.03333890026,Catagonus_wagneri:0.05154125599)Anc11:0.0315062175,Babyrousa_celebensis:0.00902322625)Anc09:0.003221719444,(Phacochoerus_africanus:0.006959393466,Potamochoerus_porcus:0.006789406534)Anc10:0.003823144618)Anc08:0.00507324458,Sus_cebifrons:0.004337171826)Anc05:0.003331364736)Anc03:0.0004324332715,Bama:0.002973816797)Anc02:0.000166612124,Ningxiang:0.002266386763)Anc01:0.0001447132373)Anc00;

In the log file, the information found in cactus_consolidated suggests that the inferred ancestor nodes are from Anc16, Anc15, Anc13, 12, 11, 9, 8, 5, 3, followed by Anc02, Anc01, and Anc00.

less nohup_output-mask.txt |grep Species [2025-01-09T19:07:25+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc07): Species tree: ((Norwegian_Landrace:0.001387818346,Duroc:0.001382871654)Anc06:0.0001757475742,(Pietrain:0.001392569191,Large_White:0.001043390809)Anc07:0.0002192699258)Anc04; [2025-01-09T22:00:20+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc14): Species tree: (((Bos_taurus:0.02936181111,Ovis_aries:0.03196458889)Anc14:0.11221609564,Babyrousa_celebensis:0.00902322625)Anc09:0.008294964024,Sus_cebifrons:0.004337171826)Anc05; [2025-01-10T17:27:42+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc06): Species tree: ((Norwegian_Landrace:0.001387818346,Duroc:0.001382871654)Anc06:0.0001757475742,(Pietrain:0.001392569191,Large_White:0.001043390809)Anc07:0.0002192699258)Anc04; [2025-01-10T19:56:25+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc10): Species tree: (Large_White:0.0025640439868,((Phacochoerus_africanus:0.006959393466,Potamochoerus_porcus:0.006789406534)Anc10:0.008896389198,Sus_cebifrons:0.004337171826)Anc05:0.003331364736)Anc03; [2025-01-11T02:28:05+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc17): Species tree: (((Canis_lupus:0.06159129412,Felis_catus:0.06412370588)Anc17:0.020181391667,Homo_sapiens:0.07980748333)Anc15:0.07427985951800001,Babyrousa_celebensis:0.00902322625)Anc09; [2025-01-12T16:49:22+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc04): Species tree: (Chenghua:0.002007248921,Meishan:0.001989041079,(Anc06:0.0001757475742,Anc07:0.0002192699258)Anc04:0.0020451418848)Anc00; [2025-01-12T20:10:50+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc16): Species tree: (((Anc17:0.014393375,Oryctolagus_cuniculus:0.099973625)Anc16:0.005788016667,Homo_sapiens:0.07980748333)Anc15:0.009434741758,Anc14:0.04737097788)Anc12; [2025-01-15T02:10:43+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc15): Species tree: (((Anc16:0.005788016667,Homo_sapiens:0.07980748333)Anc15:0.009434741758,Anc14:0.04737097788)Anc12:0.068066837204,Anc10:0.003823144618)Anc08; [2025-01-16T17:44:50+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc13): Species tree: (((Anc15:0.003457919643,Mus_musculus:0.1035495804)Anc13:0.005976822115,Anc14:0.04737097788)Anc12:0.068066837204,Anc10:0.003823144618)Anc08; [2025-01-18T12:40:30+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc12): Species tree: (((Anc13:0.005976822115,Anc14:0.04737097788)Anc12:0.06484511776,Babyrousa_celebensis:0.00902322625)Anc09:0.003221719444,Anc10:0.003823144618)Anc08; [2025-01-19T10:04:19+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc11): Species tree: (((Anc12:0.03333890026,Catagonus_wagneri:0.05154125599)Anc11:0.0315062175,Babyrousa_celebensis:0.00902322625)Anc09:0.003221719444,Anc10:0.003823144618)Anc08; [2025-01-20T07:07:18+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc09): Species tree: (((Anc11:0.0315062175,Babyrousa_celebensis:0.00902322625)Anc09:0.003221719444,Anc10:0.003823144618)Anc08:0.00507324458,Sus_cebifrons:0.004337171826)Anc05; [2025-01-20T17:13:55+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc08): Species tree: (Anc04:0.001301383252,((Anc09:0.003221719444,Anc10:0.003823144618)Anc08:0.00507324458,Sus_cebifrons:0.004337171826)Anc05:0.003331364736)Anc03; [2025-01-20T22:54:55+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc05): Species tree: (Meishan:0.001989041079,(Anc04:0.001301383252,(Anc08:0.00507324458,Sus_cebifrons:0.004337171826)Anc05:0.003331364736)Anc03:0.0007437586328000001)Anc00; [2025-01-21T06:12:27+0800] [MainThread] [I] [toil-rt] cactus_consolidated(Anc03): Species tree: (Chenghua:0.002007248921,Meishan:0.001989041079,(Anc04:0.001301383252,Anc05:0.003331364736)Anc03:0.0007437586328000001)Anc00;
I would like to ask whether Anc00 is considered the earliest ancestor node for all species. However, what I actually need is the ancestor gene sequences for the following species: Meishan, Chenghua, Ningxiang, Bama, Duroc, Norwegian Landrace, Large White, and Pietrain. Can I use the sequence from the Anc03 node as the ancestral state for the aforementioned species?

Thanks for your help.