diff --git a/internal_use/docs/source/BeginnerSegmentation/CPbeginner_Segmentation.md b/internal_use/docs/source/BeginnerSegmentation/CPbeginner_Segmentation.md index 6a7c513..010858e 100644 --- a/internal_use/docs/source/BeginnerSegmentation/CPbeginner_Segmentation.md +++ b/internal_use/docs/source/BeginnerSegmentation/CPbeginner_Segmentation.md @@ -182,8 +182,6 @@ When you are done developing your pipeline, you can exit the `'Test Mode'` by cl > **AIM: use the nuclear channel to segment (isolate and identify all the pixels belonging to) each nuclei.** -- Enter '`Test Mode'` by clicking on .\ - - Add an **IdentifyPrimaryObjects** module to the pipeline. Do this by clicking on the button in the bottom left corner of the CellProfiler window, which will pop up a small window called `‘Add modules’`. Navigate to the `Object Processing` category and select **IdentifyPrimaryObjects**. Double click on the module or click on . > **Tip**: You can also use the `'Find Modules'` search bar at the top of the ‘Add modules’ window to search all modules by name. @@ -225,6 +223,8 @@ When you are done developing your pipeline, you can exit the `'Test Mode'` by cl - Adjust the threshold method, may lead to better (or worse!) results. - Adjust the declumping settings. + - Enter '`Test Mode'` by clicking on .\ + - Hit after each change to rerun the module and see how the new parameters affect the segmentation. - Adjust the segmentation parameters until you feel you’re ready to move on to identifying the cells around the nuclei.