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Hello, I've run in to a situation that I can not wrap my head around and was hoping for some insight.
I've simulated reads for this single contig assembly using art_illumina. It is completely error free and has 100% coverage with 60,000 reads, 30,000 pairs.
When aligning with hisat2, the output SAM is as I would expect it. 60,000 pairs perfectly aligned.
However, when I try to quantify the output from hisat2 through salmon, exactly one half of reads are discarded. Every pair loses one of its reads resulting in 30,000 unpaired reads.
Hello, I've run in to a situation that I can not wrap my head around and was hoping for some insight.
I've simulated reads for this single contig assembly using art_illumina. It is completely error free and has 100% coverage with 60,000 reads, 30,000 pairs.
When aligning with hisat2, the output SAM is as I would expect it. 60,000 pairs perfectly aligned.
However, when I try to quantify the output from hisat2 through salmon, exactly one half of reads are discarded. Every pair loses one of its reads resulting in 30,000 unpaired reads.
salmon quant --libType A --alignments single_aligned.sam --targets single.fa --sampleOut --output ./
I don't understand why this is happening. If there's a safe way to upload the .fa/.fq I would do that.
Example Reads
Geneious
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